anti collagen vi rabbit 10ab (Biosynth Carbosynth)
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Anti Collagen Vi Rabbit 10ab, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+vi/Collagen+Type+VI+antibody/pmc12914450-297-15-19
Average 94 stars, based on 59 article reviews
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Incubation:Article Title: Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis. Article Snippet: For antibodies raised in mouse, Mouse on Mouse blocking reagent (Vector Laboratories) was used according to manufacturer’s instructions. .. Sections were incubated in primary antibody in PBS at 4 °C overnight with the following antibodies: collagen I (CL50151AP-1; 1:250; Cedarlane Labs), collagen IV (AB19808; 1:250; Abcam), Article Title: Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis Article Snippet: For antibodies raised in mouse, Mouse on Mouse blocking reagent (Vector Laboratories) was used according to manufacturer’s instructions. .. Sections were incubated in primary antibody in PBS at 4 °C overnight with the following antibodies: collagen I (CL50151AP-1; 1:250; Cedarlane Labs), collagen IV (AB19808; 1:250; Abcam), Article Title: Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage. Article Snippet: Following the incubation, cartilage was embedded in optimal cutting temperature (OCT) media, and unfixed, 8 μm-thick sections were obtained via Kawamoto’s film-assisted cryo-sectioning.15 The sections were rinsed with 1× PBS to remove OCT and blocked with 10% goat serum (Life Technologies) for 20 min at room temperature. .. The sections were first fluorescently labeled with the primary antibody of Article Title: Impacts of aging on murine cartilage biomechanics and chondrocyte in situ calcium signaling. Article Snippet: .. For IF imaging, paraffin sections were treated with 0.1 % pepsin (P7000, Sigma) for 20 min at 37 ◦C for antigen retrieval and incubated with primary antibodies for Sequencing:Article Title: Prevalence, parameters, and pathogenic mechanisms for splice-altering acceptor variants that disrupt the AG exclusion zone. Article Snippet: .. Whole-exome sequencing (WES), whole-genome sequencing (WGS), and muscle RNA sequencing (RNA-seq) was performed and analyzed at the Broad Institute of Harvard and MIT, as previously described.4 Immunohistochemistry (IHC) was performed as previously described.31 Antibodies to the following proteins were used: spectrin (NCL-SPEC1; dilution 1:200; Leica Microsystems) with Alexa Fluor555 conjugated goat anti-mouse secondary antibody (dilution 1:300; Thermo Fisher Scientific); Whole Complete genome sequencing:Article Title: Prevalence, parameters, and pathogenic mechanisms for splice-altering acceptor variants that disrupt the AG exclusion zone. Article Snippet: .. Whole-exome sequencing (WES), whole-genome sequencing (WGS), and muscle RNA sequencing (RNA-seq) was performed and analyzed at the Broad Institute of Harvard and MIT, as previously described.4 Immunohistochemistry (IHC) was performed as previously described.31 Antibodies to the following proteins were used: spectrin (NCL-SPEC1; dilution 1:200; Leica Microsystems) with Alexa Fluor555 conjugated goat anti-mouse secondary antibody (dilution 1:300; Thermo Fisher Scientific); RNA Sequencing:Article Title: Prevalence, parameters, and pathogenic mechanisms for splice-altering acceptor variants that disrupt the AG exclusion zone. Article Snippet: .. Whole-exome sequencing (WES), whole-genome sequencing (WGS), and muscle RNA sequencing (RNA-seq) was performed and analyzed at the Broad Institute of Harvard and MIT, as previously described.4 Immunohistochemistry (IHC) was performed as previously described.31 Antibodies to the following proteins were used: spectrin (NCL-SPEC1; dilution 1:200; Leica Microsystems) with Alexa Fluor555 conjugated goat anti-mouse secondary antibody (dilution 1:300; Thermo Fisher Scientific); Immunohistochemistry:Article Title: Prevalence, parameters, and pathogenic mechanisms for splice-altering acceptor variants that disrupt the AG exclusion zone. Article Snippet: .. Whole-exome sequencing (WES), whole-genome sequencing (WGS), and muscle RNA sequencing (RNA-seq) was performed and analyzed at the Broad Institute of Harvard and MIT, as previously described.4 Immunohistochemistry (IHC) was performed as previously described.31 Antibodies to the following proteins were used: spectrin (NCL-SPEC1; dilution 1:200; Leica Microsystems) with Alexa Fluor555 conjugated goat anti-mouse secondary antibody (dilution 1:300; Thermo Fisher Scientific); Labeling:Article Title: Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage. Article Snippet: Following the incubation, cartilage was embedded in optimal cutting temperature (OCT) media, and unfixed, 8 μm-thick sections were obtained via Kawamoto’s film-assisted cryo-sectioning.15 The sections were rinsed with 1× PBS to remove OCT and blocked with 10% goat serum (Life Technologies) for 20 min at room temperature. .. The sections were first fluorescently labeled with the primary antibody of Biomarker Discovery:Article Title: Biomimetic Proteoglycans Strengthen the Pericellular Matrix of Normal and Osteoarthritic Human Cartilage. Article Snippet: Following the incubation, cartilage was embedded in optimal cutting temperature (OCT) media, and unfixed, 8 μm-thick sections were obtained via Kawamoto’s film-assisted cryo-sectioning.15 The sections were rinsed with 1× PBS to remove OCT and blocked with 10% goat serum (Life Technologies) for 20 min at room temperature. .. The sections were first fluorescently labeled with the primary antibody of Imaging:Article Title: Impacts of aging on murine cartilage biomechanics and chondrocyte in situ calcium signaling. Article Snippet: .. For IF imaging, paraffin sections were treated with 0.1 % pepsin (P7000, Sigma) for 20 min at 37 ◦C for antigen retrieval and incubated with primary antibodies for |
